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Standardization of a hyphal inoculum of aspergilli for amphotericin B susceptibility testing.

Authors
  • V Bezjak
Publication Date
Apr 01, 1985
Source
PMC
Keywords
License
Unknown

Abstract

Standardized, evenly dispersed hyphal suspensions served as the inoculum in a microtiter technique for amphotericin B antifungal susceptibility testing. Preliminary testing with six strains of Aspergillus fumigatus and A. flavus produced consistent and reproducible results at 30 degrees C over 24 h. The observed amphotericin B MICs required for hyphae (0.3 to 0.6 microgram/ml) were comparable to MICs required for conidia (0.16 to 0.6 microgram/ml). The results were evaluated and compared with previously published information.

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