The present study aimed to standardize isotonic (IT) and isokinetic (IK) eccentric exercises by equalizing the amount of work and the angular velocity at two intensity levels, to be able to compare specific effects of these exercise modes on the neuromuscular system. Fourteen subjects participated in three test sessions consisting of two IT and two IK sets on a customized isokinetic dynamometer. IT sets were comprised of 8 eccentric contractions of the knee extensors at 120% of the maximal repetition (1RM) in the first two sessions, and 100% in the third session. IK sets were performed at the same mean angular IT velocity and stopped when the amount of work performed corresponded to the IT set. External work, angular velocity and Root Mean Square (RMS) of electromyographic activity of three superficial muscles of the quadriceps femoris were calculated. Results showed concordance of work and angular velocity for each test session. Both modes involved the same number of repetitions at 120% and fewer repetitions in IK mode at 100% of 1RM. Work and RMS values remained steady in all sets. This study allowed the standardization of isotonic and isokinetic eccentric exercises, a first step before determining their specific effects on neuromuscular function.