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Standardization of fluorescence spectra and the calibration of spectrofluorimeters.

Authors
  • Eastman, J W
Type
Published Article
Journal
Applied optics
Publication Date
Jul 01, 1966
Volume
5
Issue
7
Pages
1125–1132
Identifiers
DOI: 10.1364/AO.5.001125
PMID: 20049033
Source
Medline
License
Unknown

Abstract

A technique is proposed that can be used to calibrate the amplitude of fluorescence spectra so that spectra reported by different laboratories can be compared quantitatively. The technique is based upon the scattering of light by a solution of polystyrene. In order to compare quantitatively data obtained from different spectrofluorimeters, these terms are defined: the spectrofluorimetric sensitivity, the noise-equivalent concentration, and the fluorophotometric reproducibility. The author shows how these quantities can be measured with the polystyrene standard.

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