Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study

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Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study

Publisher
BioMed Central
Publication Date
Dec 12, 2006
Source
PMC
Keywords
Disciplines
  • Design
  • Medicine
License
Unknown

Abstract

1471-2172-7-29.fm ral ss BioMed CentBMC Immunology Open AcceMethodology article Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study Wayne G Shreffler1, Cynthia M Visness2, Melissa Burger3, William W Cruikshank4, Howard M Lederman5, Maite de la Morena6, Kristine Grindle3, Agustin Calatroni2, Hugh A Sampson1 and James E Gern*3 Address: 1Mount Sinai School of Medicine, Division of Pediatric Allergy & Immunology, New York, NY, USA, 2Rho Inc., Chapel Hill, NC, USA, 3Department of Pediatrics, University of Wisconsin Hospital, Madison, WI, USA, 4Pulmonary Center, Boston University School of Medicine, Boston, MA, USA, 5Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA and 6Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA Email: Wayne G Shreffler - [email protected]; Cynthia M Visness - [email protected]; Melissa Burger - [email protected]; William W Cruikshank - [email protected]; Howard M Lederman - [email protected]; Maite de la Morena - [email protected]; Kristine Grindle - [email protected]; Agustin Calatroni - [email protected]; Hugh A Sampson - [email protected]; James E Gern* - [email protected] * Corresponding author Abstract Background: Cryopreservation of peripheral blood mononuclear cells has been used to preserve and standardize immunologic measurements for multicenter studies, however, effects of cryopreservation on cytokine responses are incompletely understood. In designing immunologic studies for a new multicenter birth cohort study of childhood asthma, we performed a series of experiments to determine the effects of two different methods of cryopreservation on the cytokine responses of cord and peripheral blood mononuclear cells. Results: Paired samples of PBMC were processed freshly, or after cryopreservation in a Nalgene container (NC) or a controlled-r

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