Affordable Access

Staining of DNA with uranylacetate in hydrolysed ultrathin sections.

Authors
Type
Published Article
Journal
Acta Histochemica
0065-1281
Publisher
Elsevier
Publication Date
Volume
68
Issue
1
Pages
22–26
Identifiers
PMID: 6167127
Source
Medline
License
Unknown

Abstract

Rat liver and hepatoma cells fixed with formaldehyde, embedded into Epon and treated on sections with 5 n HCl and then with aqueous uranylacetate show preferential DNase-sensitive reaction. The reaction is highly dependent upon proper fixation, hydrolysis improves its specificity. The binding of the contrast with DNA is of ionic nature. Because of its simplicity, sufficient contrast and resolution the suggested technique is recommended for ultrastructural studies of DNA-containing substrates.

Statistics

Seen <100 times