Staining of DNA with uranylacetate in hydrolysed ultrathin sections.
- Published Article
- Publication Date
Jan 01, 1981
Rat liver and hepatoma cells fixed with formaldehyde, embedded into Epon and treated on sections with 5 n HCl and then with aqueous uranylacetate show preferential DNase-sensitive reaction. The reaction is highly dependent upon proper fixation, hydrolysis improves its specificity. The binding of the contrast with DNA is of ionic nature. Because of its simplicity, sufficient contrast and resolution the suggested technique is recommended for ultrastructural studies of DNA-containing substrates.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/6167127