Affordable Access

deepdyve-link
Publisher Website

Spontaneous superimposed preeclampsia: chronology and expression unveiled by temporal transcriptomic analysis.

Authors
  • Maeda, Kenji J1
  • Showmaker, Kurt C1, 2
  • Johnson, Ashley C1, 2
  • Garrett, Michael R1, 2, 3
  • Sasser, Jennifer M1
  • 1 Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi.
  • 2 Molecular and Genomics Core, University of Mississippi Medical Center, Jackson, Mississippi.
  • 3 Department of Medicine (Nephrology), University of Mississippi Medical Center, Jackson, Mississippi.
Type
Published Article
Journal
Physiological Genomics
Publisher
American Physiological Society
Publication Date
Aug 01, 2019
Volume
51
Issue
8
Pages
342–355
Identifiers
DOI: 10.1152/physiolgenomics.00020.2019
PMID: 31125289
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Preeclampsia (PE), a multifactorial pregnancy-specific syndrome accounting for up to 8% of pregnancy complications, is a leading cause of maternal and fetal morbidity and mortality. PE is also associated with long-term risk of hypertension and stroke for both mother and fetus. Currently, the only "cure" is delivery of the baby and placenta, largely because the pathogenesis of PE is not yet fully understood. PE is associated with impaired vascular remodeling at the maternal-fetal interface and placental insufficiency; however, specific factors contributing to this impairment have not been identified. To identify molecular pathways involved in PE, we examined temporal transcriptomic changes occurring within the uterus, uterine implantation sites, and placentae from the Dahl salt-sensitive (Dahl S) rat model of superimposed PE compared with Sprague Dawley (SD) rats. We hypothesized that targeted gene analysis and whole transcriptome analysis would identify genetic factors that contribute to development of the preeclamptic phenotype in the Dahl S rat and unveil novel biomarkers, therapeutic targets, and mechanistic pathways in PE. Quantitative real-time PCR (qRT-PCR) and whole genome microarray analysis were performed on isolated total RNA from uterus (day 0), uterine implantation sites (days 7 and 10), and placenta (days 14 and 20). We found 624, 332, 185, and 366 genes to be differentially expressed between Dahl S (PE) and SD (normal pregnancy) on days 0, 7, 10, and 14, respectively. Our data revealed numerous pathways that may play a role in the pathophysiology of spontaneous superimposed PE and allow for further investigation of novel therapeutic targets and biomarker development.

Report this publication

Statistics

Seen <100 times