Affordable Access

Spontaneous structural changes in DNA during fibroblast aging and the establishment process in vitro.

Authors
  • Icard, C
  • Beaupain, R
Type
Published Article
Journal
Mechanisms of Ageing and Development
Publisher
Elsevier
Publication Date
Jan 01, 1980
Volume
14
Issue
1-2
Pages
81–87
Identifiers
PMID: 7206817
Source
Medline
License
Unknown

Abstract

The molecular weight of single-stranded DNA isolated from human fibroblasts decreased in phase III by comparison with phase II. Mouse fibroblast DNA isolated during the growth crisis had a decreased molecular weight compared to the initial DNA. Established mouse cells recovered this high molecular weight DNA. Cells treated with caffeine during the growth crisis did not survive while established cells were resistant to the same conditions of caffeine treatment. A DNA repair process may play a role in establishing a permanent cell line.

Report this publication

Statistics

Seen <100 times