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Spontaneous spectinomycin resistance mutations detected after biolistic transformation of Daucus carota L.

Authors
  • Filipenko, Elena A
  • Sidorchuk, Yuri V
  • Titov, Igor I
  • Maltsev, Valery P
  • Deineko, Elena V
Type
Published Article
Journal
Physiology and molecular biology of plants : an international journal of functional plant biology
Publication Date
Mar 01, 2011
Volume
17
Issue
1
Pages
79–86
Identifiers
DOI: 10.1007/s12298-011-0051-0
PMID: 23572997
Source
Medline
Keywords
License
Unknown

Abstract

Spectinomycin resistant mutant carrot (Daucus carota L.) callus lines detected in the experiments on biolistic transformation of plastome were analyzed. It has been found that this antibiotic resistance is determined by point nucleotide substitutions at two distinct sites of the chloroplast gene rrn16, coding for 16S rRNA, namely, G1012T, G1012C, and A1138G. The detected mutations are localized to the 16S rRNA region forming helix h34, which contains spectinomycin binding site, and lead to its destabilization by several kilocalories per mole. Comparative analysis of rrn16 gene sequences has demonstrated conservation of the positions of the nucleotide substitutions determining this antibiotic resistance in carrot (D. carota L.), tobacco (Nicotiana tabacum L.), and bladder pod (Lesquerella fendleri L.), as well as in Escherichia coli.

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