Affordable Access

Access to the full text

Split Green Fluorescent Proteins: Scope, Limitations, and Outlook

Authors
  • Romei, Matthew G.
  • Boxer, Steven G.
Type
Published Article
Journal
Annual Review of Biophysics
Publisher
Annual Reviews
Publication Date
May 06, 2019
Volume
48
Pages
19–44
Identifiers
DOI: 10.1146/annurev-biophys-051013-022846
Source
Annual Reviews
Keywords
License
Yellow

Abstract

Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein–protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.

Report this publication

Statistics

Seen <100 times