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SpeedScreen: The "missing link" between genomics and lead discovery.

Authors
  • Zehender, Hartmut
  • Le Goff, Francois
  • Lehmann, Natalie
  • Filipuzzi, Ireos
  • Mayr, Lorenz M
Type
Published Article
Journal
Journal of Biomolecular Screening
Publisher
SAGE Publications
Publication Date
Sep 01, 2004
Volume
9
Issue
6
Pages
498–505
Identifiers
PMID: 15452336
Source
Medline
License
Unknown

Abstract

SpeedScreen is a novel, label-free, in-solution, affinity-based selection methodology for high-throughput screening (HTS) developed at Novartis Pharma. The SpeedScreen protocol comprises in-solution affinity selection, followed by size exclusion chromatography in combination with microbore-liquid-chromatography/electrospray-ionization mass spectrometry (micro-LC/ESI-MS). The authors describe the basic concept behind assay development, HTS, and data analysis with the SpeedScreen technology. Advantages and limitations of SpeedScreen compared to alternative screening technologies are discussed, and an example is given from a SpeedScreen campaign applying this innovative affinity selection concept in HTS.

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