Affordable Access

Spectroscopic probe of distribution of the non-intercalating drug netropsin between DNA and heparin.

Authors
  • Pal, M K
  • Ghosh, J K
Type
Published Article
Journal
Indian journal of biochemistry & biophysics
Publication Date
Aug 01, 1992
Volume
29
Issue
4
Pages
336–338
Identifiers
PMID: 1330895
Source
Medline
License
Unknown

Abstract

Circular dichroism has been used as a monitoring tool to probe the distribution of the non-intercalating drug netropsin (NTPS) between the two biomolecules DNA and heparin. The stoichiometry of the interaction of the individual biomolecules and the drug is determined from conductometric titrations; the titration in each case shows two breaks corresponding to two stoichiometries of interaction. Though netropsin is non-intercalating, DNA wins over heparin in binding the drug due to strong hydrogen bonding capability of NTPS in the minor grooves of DNA through its greater than NH donor groups. Potential hydrogen bond breakers like KF and urea reduce the induced dichroism of NTPS-DNA system, probably dislodging some drug from DNA through hydrogen bond breaking.

Report this publication

Statistics

Seen <100 times