The spectra produced by the acid treatment and alkaline treatment of streptomycin and dihydrostreptomycin have been investigated to obtain suitable methods for their determination. Mixtures of the two antibiotics have been successfully determined in the UV region. Thus, using the orthogonal function method, dihydrostreptomycin has been determined after acid treatment whereas streptomycin has been determined after alkaline treatment followed by immediate neutralization.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/76315