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Spectrophotometric analysis of flavonoid-DNA binding interactions at physiological conditions.

Authors
  • Janjua, Naveed Kausar1
  • Siddiqa, Asima
  • Yaqub, Azra
  • Sabahat, Sana
  • Qureshi, Rumana
  • ul Haque, Sayed
  • 1 Department of Chemistry, Quaid-i-Azam University, Islamabad 45230, Pakistan. [email protected] , (Pakistan)
Type
Published Article
Journal
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Publication Date
December 2009
Volume
74
Issue
5
Pages
1135–1137
Identifiers
DOI: 10.1016/j.saa.2009.09.022
PMID: 19836298
Source
Medline
License
Unknown

Abstract

Mode of interactions of three flavonoids [morin (M), quercetin (Q), and rutin (R)] with chicken blood ds.DNA (ck.DNA) has been investigated spectrophotometrically at different temperatures including body temperature (310 K) and at two physiological pH values, i.e. 7.4 (human blood pH) and 4.7 (stomach pH). The binding constants, K(f), evaluated using Benesi-Hildebrand equation showed that the flavonoids bind effectively through intercalation at both pH values and body temperature. Quercetin, somehow, showed greater binding capabilities with DNA. The free energies of flavonoid-DNA complexes indicated the spontaneity of their binding. The order of binding constants of three flavonoids at both pH values were found to be K(f(Q)) > K(f(R)) > K(f(M)) and at 310 K.

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