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Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.

Authors
  • Wasylyk, B
  • Derbyshire, R
  • Guy, A
  • Molko, D
  • Roget, A
  • Téoule, R
  • Chambon, P
Type
Published Article
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Publication Date
Dec 01, 1980
Volume
77
Issue
12
Pages
7024–7028
Identifiers
PMID: 6938951
Source
Medline
License
Unknown

Abstract

A single-point mutation, consisting of a T-to-G transversion, was made in the third nucleotide of the conalbumin gene T-A-T-A-A-A-A homology sequence (the T-A-T-A or Goldberg-Hogness box). In an in vitro system, specific transcription of the mutant DNA was drastically decreased compared to the normal gene. This down-mutation is consistent with the idea that the T-A-T-A box is an important element for specific initiation of transcription.

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