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Specific receptors for the serum thymic factor (FTS) in lymphoblastoid cultured cell lines.

Authors
  • Pléau, J M
  • Fuentes, V
  • Morgat, J L
  • Bach, J F
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
May 01, 1980
Volume
77
Issue
5
Pages
2861–2865
Identifiers
PMID: 6248873
Source
Medline
License
Unknown

Abstract

The interaction of the synthetic serum thymic factor (FTS, "facteur thymique sérique") with human T lymphocytes from two established T cell lines (1301 and HSB2) was studied by using [3H]FTS. At 30 degrees C, in the presence of 10 nM labeled FTS, a steady state was observed within 120 min. Bound labeled FTS was dissociated by simultaneous addition of an excess of unlabeled FTS (dissociation constant Kd = 3 nM). The binding of [3H]FTS was reduced to 10-20% when unlabeled FTS was added at 20 microgram/ml (20 micro M), but not when unrelated peptides or FTS analogues differing from FTS by only one amino acid residue were added. No FTS binding was detectable on B and null cell lines or on two T cell lines other than 1301 and HSB2. These data indicate that only certain T cell subsets possess receptors with high affinity and specificity for FTS.

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