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Spatiotemporally Non-Uniform Ca2+ Dynamics of Cardiac Purkinje Fibers in Mouse Myocardial Infarct.

Authors
  • Matsuyama, Taka-Aki1
  • Tanaka, Hideo1
  • Ishibashi-Ueda, Hatsue2
  • Takamatsu, Tetsuro3
  • 1 Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kyoto, Japan. , (Japan)
  • 2 Department of Pathology, National Cerebral and Cardiovascular Center, Suita, Osaka, Japan. , (Japan)
  • 3 Department of Medical Photonics, Kyoto Prefectural University of Medicine, Kyoto, Japan. , (Japan)
Type
Published Article
Journal
Journal of Histochemistry & Cytochemistry
Publisher
SAGE Publications
Publication Date
Nov 01, 2017
Volume
65
Issue
11
Pages
655–667
Identifiers
DOI: 10.1369/0022155417730280
PMID: 28903013
Source
Medline
Keywords
License
Unknown

Abstract

Surviving Purkinje fibers in myocardial infarct are regarded as an important substrate in arrhythmogenesis. However, poorly understood are functional properties of Purkinje fibers in the infarcted heart. We sought to visualize intracellular Ca2+ ([Ca2+]i) dynamics of Purkinje fiber networks in the mouse myocardial infarct. Using 3- to 4-day-old or 7- to 9-day-old infarcted hearts after the left coronary-artery ligation corresponding, respectively, to acute or healing phase, we conducted rapid fluo4-fluorescence imaging on the endocardial surface of the left ventricular septum by macro-zoom fluorescence microscopy and rapid-scanning confocal microscopy. In contrast with the intact heart, where uniform Ca2+ transients propagated rapidly, the infarcted heart exhibited slow, non-uniform impulse propagations. On confocal microscopy, Purkinje fibers in the peri-infarct zone exhibited non-uniform [Ca2+]i dynamics: beat-to-beat alternans of the Ca2+ transient amplitude in and among the individual fibers, whereas the intact fibers exhibited uniform Ca2+ transients. Such non-uniform [Ca2+]i dynamics were more conspicuous in the acute infarcted hearts than in the healing ones. In accordance with [Ca2+]i dynamics, fixed fluo4-loaded heart preparations exhibited definitive connexin-40 plaques in the peri-infarct Purkinje fibers, whereas the subjacent myocardium presented coagulative necrosis and granulation tissues, respectively. The surviving Purkinje fibers in the peri-infarct zone exhibited non-uniform [Ca2+]i dynamics, which may lead to arrhythmogenesis.

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