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Spatiotemporal expression of UPK3B and its promoter activity during embryogenesis and spermatogenesis.

Authors
  • Kuriyama, Sei1
  • Tamiya, Yuutaro2, 3
  • Tanaka, Masamitsu2
  • 1 Department of Molecular Biochemistry, Graduate School Medicine Akita University, Hondo 1-1-1, Akita City, Akita, 010-8543, Japan. [email protected] , (Japan)
  • 2 Department of Molecular Biochemistry, Graduate School Medicine Akita University, Hondo 1-1-1, Akita City, Akita, 010-8543, Japan. , (Japan)
  • 3 Department of Lifescience, Faculty and Graduate School of Engineering and Resource Science, Akita University, 1-1 Tegata Gakuenmachi, Akita City, Akita, 010-8502, Japan. , (Japan)
Type
Published Article
Journal
Histochemistry and cell biology
Publication Date
Jan 01, 2017
Volume
147
Issue
1
Pages
17–26
Identifiers
DOI: 10.1007/s00418-016-1486-8
PMID: 27577269
Source
Medline
Keywords
License
Unknown

Abstract

Uroplakin (Upk) 3 is one of the main structural components of the urothelium tissue. Although expression of UPK3B is seen in a wider variety of the tissues and organs than UPK3A, tissue-specific expression has not yet been analyzed. Here, we analyzed the Cre recombinase activity driven by the Upk3b promoter in transgenic mice and the endogenous localization of UPK3B. We generated Tg(Upk3b-Cre)/R26tdTomato mice by crossing ROSA26tm14(CAG-tdTomato) (R26tdTomato) mice with Tg(Upk3b-Cre) mice and investigated the spatiotemporal distribution of tdTomato in embryonic and adult mice. In embryos, we detected Cre recombinase activity in neural crest cells and the heart, liver, kidneys, and lungs. In adult mice, Cre recombinase activity was detected in male and female genital organs; however, the activity was absent in the bladder. Histological analyses revealed that both tdTomato and UPK3B were present in testicular and epididymal sperm; however, tdTomato was not present in the ductus epididymis, where the endogenous expression of UPK3B was detected. In female siblings, both tdTomato and UPK3B expressions were detected in the follicles of the ovary, whereas no tdTomato expression was found in the mucosal epithelium of the fallopian tubes, where the endogenous UPK3B was expressed. These data suggest that UPK3B may play a pivotal role in the maturation of gametes and gamete-delivery organs.

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