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Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography.

Authors
  • Olefir, Ivan1
  • Ghazaryan, Ara2
  • Yang, Hong2
  • Malekzadeh-Najafabadi, Jaber2
  • Glasl, Sarah2
  • Symvoulidis, Panagiotis1
  • O'Leary, Valerie B3
  • Sergiadis, George4
  • Ntziachristos, Vasilis5
  • Ovsepian, Saak V6
  • 1 Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany; Chair of Biological Imaging, Technical University Munich, 81675 Munich, Germany. , (Germany)
  • 2 Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany. , (Germany)
  • 3 Department of Medical Genetics, Third Faculty of Medicine of Charles University, 11636 Prague, Czech Republic. , (Czechia)
  • 4 Department of Electrical and Computer Engineering, Aristotle University, 54124 Thessaloniki, Greece. , (Greece)
  • 5 Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany; Chair of Biological Imaging, Technical University Munich, 81675 Munich, Germany. Electronic address: [email protected] , (Germany)
  • 6 Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum Munich, Ingolstadter Landstrasse 1, 85764 Neuherberg, Germany; Chair of Biological Imaging, Technical University Munich, 81675 Munich, Germany; Department of Experimental Neurobiology, National Institute of Mental Health, Topolová 748, 25067 Klecany, Czech Republic; Department of Psychiatry and Medical Psychology, Third Faculty of Medicine of Charles University, 11636 Prague, Czech Republic. Electronic address: [email protected] , (Czechia)
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Mar 05, 2019
Volume
26
Issue
10
Identifiers
DOI: 10.1016/j.celrep.2019.02.020
PMID: 30840901
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In traditional optical imaging, limited light penetration constrains high-resolution interrogation to tissue surfaces. Optoacoustic imaging combines the superb contrast of optical imaging with deep penetration of ultrasound, enabling a range of new applications. We used multispectral optoacoustic tomography (MSOT) for functional and structural neuroimaging in mice at resolution, depth, and specificity unattainable by other neuroimaging modalities. Based on multispectral readouts, we computed hemoglobin gradient and oxygen saturation changes related to processing of somatosensory signals in different structures along the entire subcortical-cortical axis. Using temporal correlation analysis and seed-based maps, we reveal the connectivity between cortical, thalamic, and sub-thalamic formations. With the same modality, high-resolution structural tomography of intact mouse brain was achieved based on endogenous contrasts, demonstrating near-perfect matches with anatomical features revealed by histology. These results extend the limits of noninvasive observations beyond the reach of standard high-resolution neuroimaging, verifying the suitability of MSOT for small-animal studies. Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.

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