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Spatial Ecology of the Human Tongue Dorsum Microbiome.

Authors
  • Wilbert, Steven A1
  • Mark Welch, Jessica L2
  • Borisy, Gary G3
  • 1 The Forsyth Institute, Cambridge, MA 02139, USA.
  • 2 Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543, USA. Electronic address: [email protected]
  • 3 The Forsyth Institute, Cambridge, MA 02139, USA; Harvard School of Dental Medicine, Boston, MA 02115, USA. Electronic address: [email protected]
Type
Published Article
Journal
Cell Reports
Publisher
Elsevier
Publication Date
Mar 24, 2020
Volume
30
Issue
12
Identifiers
DOI: 10.1016/j.celrep.2020.02.097
PMID: 32209464
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

A fundamental question in microbial ecology is how microbes are spatially organized with respect to each other and their host. A test bed for examining this question is the tongue dorsum, which harbors a complex and important microbial community. Here, we use multiplexed fluorescence spectral imaging to investigate the organization of the tongue microbiome at micron to hundred-micron scales. We design oligonucleotide probes for taxa both abundant and prevalent, as determined by sequence analysis. Imaging reveals a highly structured spatial organization of microbial consortia, ranging in linear dimension from tens to hundreds of microns. The consortia appear to develop from a core of epithelial cells, with taxa clustering in domains suggestive of clonal expansion. Quantitative proximity analysis provides the basis for a model of tongue dorsum microbiome organization and dynamics. Our work illustrates how high-resolution analysis of micron-scale organization provides insights into physiological functions and microbiome-host interactions. Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

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