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Soybean trypsin inhibitor. An IL-1-like protein?

Authors
  • Richard, K A
  • Speziale, S C
  • Staite, N D
  • Berger, A E
  • Deibel, M R Jr
  • Finzel, B C
  • Einspahr, H M
Type
Published Article
Journal
Agents and Actions
Publisher
Springer-Verlag
Publication Date
Jun 01, 1989
Volume
27
Issue
3-4
Pages
265–267
Identifiers
PMID: 2801308
Source
Medline
License
Unknown

Abstract

Soybean trypsin inhibitor (SBTI) shares some structural homology with interleukin-1 (IL-1) and was tested for IL-1 bioactivity. Human T-cells proliferated maximally when stimulated with PMA and SBTI but failed to respond to either stimulus alone. This response was abrogated by neutralizing antibodies to IL-1 beta but not to IL-1 alpha. However, immunoblots showed no cross-reactivity between SBTI and anti-IL-1 antibodies. Furthermore, SBTI did not bind to IL-1 receptors on YT cells and did not activate a murine T-lymphoma or human T-hybridoma. Supernantants from monocytes stimulated with SBTI contained significant levels of IL-1 activity. The data show that SBTI has no direct IL-1 activity but can stimulate T-cells indirectly through an IL-1 dependent mechanism.

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