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Southern blotting.

Authors
Type
Published Article
Journal
Nature Protocols
1750-2799
Publisher
Nature Publishing Group
Publication Date
Volume
1
Issue
2
Pages
518–525
Identifiers
PMID: 17406277
Source
Medline

Abstract

This protocol describes a basic method to perform the Southern blot. Blotting allows the detection of specific molecules among a mixture separated by gel electrophoresis. Molecules are transferred from the gel to a porous membrane by capillary action using absorbent paper to soak solution through the gel and the membrane. For DNA, specific sequences are detected in the membrane by molecular hybridization with labeled nucleic acid probes. The original method, on which this protocol is based, used labeled RNAs to detect specific DNA fragments in genomic DNA that had been digested with restriction endonucleases. This protocol can be completed in 1-5 d and is inexpensive to carry out, as it requires only basic laboratory equipment.

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