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Sonic Hedgehog Regulates Bone Fracture Healing

Authors
  • Takebe, Hiroaki1
  • Shalehin, Nazmus1
  • Hosoya, Akihiro1
  • Shimo, Tsuyoshi
  • Irie, Kazuharu1
  • 1 (K.I.)
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
Jan 20, 2020
Volume
21
Issue
2
Identifiers
DOI: 10.3390/ijms21020677
PMID: 31968603
PMCID: PMC7013927
Source
PubMed Central
Keywords
License
Green

Abstract

Bone fracture healing involves the combination of intramembranous and endochondral ossification. It is known that Indian hedgehog (Ihh) promotes chondrogenesis during fracture healing. Meanwhile, Sonic hedgehog (Shh), which is involved in ontogeny, has been reported to be involved in fracture healing, but the details had not been clarified. In this study, we demonstrated that Shh participated in fracture healing. Six-week-old Sprague–Dawley rats and Gli-CreERT2; tdTomato mice were used in this study. The right rib bones of experimental animals were fractured. The localization of Shh and Gli1 during fracture healing was examined. The localization of Gli1 progeny cells and osterix (Osx)-positive cells was similar during fracture healing. Runt-related transcription factor 2 (Runx2) and Osx, both of which are osteoblast markers, were observed on the surface of the new bone matrix and chondrocytes on day seven after fracture. Shh and Gli1 were co-localized with Runx2 and Osx. These findings suggest that Shh is involved in intramembranous and endochondral ossification during fracture healing.

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