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Solid-Phase-Fluorimetric Determination of Quinolones in Medicinal Preparations on Cellulose Paper and in a Thin Silica Layer Using a Smartphone

Authors
  • Amelin, V. G.1, 2
  • Shogah, Z. A. Ch.1
  • Bol’shakov, D. S.2
  • 1 Vladimir State University, Vladimir, 600000, Russia , Vladimir (Russia)
  • 2 Federal Center for Animal Health, Yur’evetsVladimir, 600901, Russia , Yur’evetsVladimir (Russia)
Type
Published Article
Journal
Journal of Analytical Chemistry
Publisher
Pleiades Publishing
Publication Date
Jul 03, 2021
Volume
76
Issue
7
Pages
797–805
Identifiers
DOI: 10.1134/S1061934821070030
Source
Springer Nature
Keywords
Disciplines
  • Articles
License
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Abstract

AbstractA simple and an available method is proposed for the determination of quinolone antibiotics using solid-phase fluorescence digital colorimetry. The intrinsic fluorescence of 17 quinolones and the quinolone-sensitizedfluorescence of europium on cellulose paper (CP) and a thin silica layer are studied. Upon the irradiation of quinolone solutions applied to a matrix with ultraviolet light (365 nm), blue (CP, high-performance thin-layer chromatography (HPTLC)) and pink (CP–Eu, HPTLC–Eu) fluorescence is observed. Measurement of the fluorescence intensity on the surface of matrices is carried out using a smartphone. The values of colorimetric parameters in the RGB system are used as the analytical signal (Ar): Ar = \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\sqrt {{{{({{{\text{R}}}_{0}} - {{{\text{R}}}_{{\text{x}}}})}}^{2}} + {{{({{{\text{G}}}_{0}} - {{{\text{G}}}_{{\text{x}}}})}}^{2}} + {{{({{{\text{B}}}_{0}} - {{{\text{B}}}_{{\text{x}}}})}}^{2}}} .$$\end{document} The limits of detection and determination are 0.2–4 and 0.6–12 µg/mL, respectively, for all analyzed analytes. The analytical ranges are 0.6–500 µg/mL. A procedure for the determination of fluoroquinolones in medicinal preparations is proposed. The relative standard deviation of the results of analysis does not exceed 5%.

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