Solid-phase synthesis of O-glycosylated Nalpha-Fmoc amino acids and analysis by high-resolution magic angle spinning NMR.
- Authors
- Type
- Published Article
- Journal
- Journal of Combinatorial Chemistry
- Publisher
- American Chemical Society
- Volume
- 6
- Issue
- 2
- Pages
- 214–219
- Source
- Kit Lam Lab
- License
- Unknown
Abstract
Direct O-glycosylation of amino acids bound to TentaGel resin with a number of glycosyl trichloroacetimidate donors results in high yields. The glycosylation reaction can be easily monitored by analyzing the bead-bound amino acids with high-resolution magic angle spinning (HR-MAS) NMR. These studies pave a new way for the construction of "one-bead one-compound" O-glycopeptide libraries with standard amino acid building blocks and appropriate glycosyl trichloroacetimidate donors.
