A solid phase fluorescent immunossay for the quantitation of the C4 component of human complement.
- Published Article
Journal of Immunological Methods
- Publication Date
Jan 01, 1977
A non-competitive method for the determination of the C4 component of human complement in serum is described. This procedure involves use of a specific antibody covalently attached to derivatized polyacrylamide beads and a fluorescently labeled specific antibody. Reproducible results were achieved for C4 in serum in the range of 10 mg/dl to 170 mg/dl within 2 h. C4 levels as low as 150 ng/ml can also be measured. Fluorescent immunoassay and radial immunodiffusion were used to determine C4 levels in healthy adults. Good agreement was found between the two methods.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/406329