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A solid phase fluorescent immunossay for the quantitation of the C4 component of human complement.

Authors
  • Burgett, M W
  • Fairfield, S J
  • Monthony, J F
Type
Published Article
Journal
Journal of Immunological Methods
Publisher
Elsevier
Publication Date
Jan 01, 1977
Volume
16
Issue
3
Pages
211–219
Identifiers
PMID: 406329
Source
Medline
License
Unknown

Abstract

A non-competitive method for the determination of the C4 component of human complement in serum is described. This procedure involves use of a specific antibody covalently attached to derivatized polyacrylamide beads and a fluorescently labeled specific antibody. Reproducible results were achieved for C4 in serum in the range of 10 mg/dl to 170 mg/dl within 2 h. C4 levels as low as 150 ng/ml can also be measured. Fluorescent immunoassay and radial immunodiffusion were used to determine C4 levels in healthy adults. Good agreement was found between the two methods.

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