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A solid phase enzyme-linked immunosurbent assay (ELISA) for the demonstration of antibodies against denatured, single-stranded DNA in patient sera.

Authors
  • Gripenberg, M
  • Linder, E
  • Kurki, P
  • Engvall, E
Type
Published Article
Journal
Scandinavian journal of immunology
Publication Date
Jan 01, 1978
Volume
7
Issue
2
Pages
151–157
Identifiers
PMID: 349676
Source
Medline
License
Unknown

Abstract

A solid phase enzyme-linked-immunosorbent assay (ELISA) for the determination of antibodies against denatured, single-stranded (ss-) DNA is described. Polystyrene cuvettes coated with ss-DNA were incubated with serum samples and the anti-ss-DNA antibodies bound were detected by means of an anti-IgG-alkaline phosphatase conjugate. The binding of anti-ss-DNA antibodies in individual sera was expressed as units calculated as % of the absorbance in relation to the absorbance value obtained with a reference pool. Absorption experiments showed that the assay is specific for antibodies against denatured DNA. By using immunologically purified anti-ss-DNA antibodies the assay was shown to detect specific antibodies in concentrations down to 1 ng/ml. Antibodies against DNA could be detected in 94% of sera with antinuclear antibodies.

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