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Sol-gel encapsulation of lactate dehydrogenase for optical sensing of L-lactate.

Authors
  • Li, Chia I
  • Lin, Yi Hua
  • Shih, Cheng Ling
  • Tsaur, Jeng Pyng
  • Chau, Lai Kwan
Type
Published Article
Journal
Biosensors and Bioelectronics
Publisher
Elsevier
Publication Date
Apr 01, 2002
Volume
17
Issue
4
Pages
323–330
Identifiers
PMID: 11849929
Source
Medline
License
Unknown

Abstract

Sol-gel encapsulation of lactate dehydrogenase and its cofactor can be employed as a disposable sensor for L-lactate. The sensor utilized the changes in absorbance or fluorescence from the reduced cofactor nicotinamide adenine dinucleotide (NADH) upon exposure to L-lactate. Although, problems such as diminished enzymatic activity and/or leaching of enzyme from the sol-gel matrix occurred, the sol-gel process is sufficiently mild to permit retention of enzymatic activity. The apparent activity of LDH in the sensor is at least 10% of that of the dissolved enzyme. The sensor has a linear dynamic range over the normal physiological L-lactate level and has a long-term storage stability of at least 3 weeks.

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