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A smooth muscle cell line suitable for the study of voltage sensitive calcium channels.

Authors
  • Rüegg, U T
  • Doyle, V M
  • Zuber, J F
  • Hof, R P
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Jul 16, 1985
Volume
130
Issue
1
Pages
447–453
Identifiers
PMID: 2411261
Source
Medline
License
Unknown

Abstract

A cell line originating from the fetal rat aorta has been studied with respect to 45Ca2+ uptake. Kinetic experiments showed an initial rapid uptake followed by a slow linear phase; both the initial rate and the maximum uptake were increased in the presence of 55 mM potassium chloride. The calcium channel antagonists, darodipine (PY 108-068) and verapamil, inhibited both the basal and the potassium chloride stimulated uptake. Neither tetrodotoxin nor furosemide affected either basal or depolarisation induced 45Ca2+ uptake. Blockade of the Na+/K+ ATPase by ouabain and of the Ca2+ ATPase by vanadate caused a net increase in cellular 45Ca2+ accumulation.

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