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Smartphone operable centrifugal system (SOCS) for on-site DNA extraction from foodborne bacterial pathogen

Authors
  • Jeong, Soon Woo1
  • Park, Yoo Min1
  • Jo, Sung Hee1
  • Lee, Seok Jae1
  • Kim, Yong Tae2
  • Lee, Kyoung G.1
  • 1 Nano-Bio Application Team, National Nanofab Center, South Korea
  • 2 Department of Chemical Engineering & Biotechnology, Korea Polytechnic University, South Korea
Type
Published Article
Journal
Biomicrofluidics
Publisher
American Institute of Physics
Publication Date
May 22, 2019
Volume
13
Issue
3
Identifiers
DOI: 10.1063/1.5093752
PMID: 31149321
PMCID: PMC6531255
Source
PubMed Central
License
Unknown

Abstract

The on-site recovery of nucleic acid from foodborne bacteria is in high demand to further understand on-site molecular diagnosis, which is especially applicable in developing countries. Here, we first proposed a smartphone operable centrifugal system (SOCS) for nucleic acid extraction with the assistance of a low power consumable motor and hydrogel beads. The SOCS consists of a centrifugal motor, 3D-printed cartridge, a nucleic acid collection column, and a smartphone. The SOCS shows excellent DNA extraction performance within 6 min, and it can operate more than 100 times using a smartphone. The purified effluent DNA was accumulated in the nucleic acid collection column. The performance of the SOCS was confirmed by amplifying the recovered DNA from Escherichia coli O157:H7. Moreover, the artificially inoculated food and blood samples also confirmed the performance of SOCS. The proposed SOCS provides an on-site operable nucleic acid separation platform in terms of simplicity, easy usability, cost-effectiveness, and portability in pathogenic point-of-care diagnostics.

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