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The smaller isoform of the mitochondrial transcription factor A has a role in the mitochondrial transcription.

Authors
  • Bruno, Stefano
  • De Virgilio, Caterina
  • Gadaleta, Gemma
Type
Published Article
Journal
The Italian journal of biochemistry
Publication Date
Dec 01, 2007
Volume
56
Issue
4
Pages
315–318
Identifiers
PMID: 19192634
Source
Medline
License
Unknown

Abstract

The mitochondrial transcription factor A (Tfam) is a mitochondrial protein encoded in the nucleus. The gene for Tfam spans about 10 kb and consists of seven exons and six introns. In human and rat, exon 5 can splite alternatively resulting in two Tfam isoforms. In order to investigate the role of the delta 5Tfam isoform in human cells, we studied its stability in vitro, then we carried out overexpression experiments in H1299 human cell line in order to clarify the in vivo effect of this shorter isoform of Tfam. The data obtained by Real time-PCR demonstrate that the overexpression of delta 5Tfam causes an increase of mitochondrial transcription, so also this isoform as a role in the mitochondrial process.

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