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Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes.

Authors
  • Carballar-Lejarazú, Rebeca1
  • Pham, Thai Binh2
  • Bottino-Rojas, Vanessa1
  • Adolfi, Adriana3
  • James, Anthony A4
  • 1 Department of Microbiology & Molecular Genetics, University of California, Irvine.
  • 2 Department of Molecular Biology & Biochemistry, University of California, Irvine.
  • 3 Department of Microbiology & Molecular Genetics, University of California, Irvine; Vector Biology Department, Liverpool School of Tropical Medicine.
  • 4 Department of Microbiology & Molecular Genetics, University of California, Irvine; Department of Molecular Biology & Biochemistry, University of California, Irvine; [email protected]
Type
Published Article
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corporation
Publication Date
May 01, 2021
Issue
171
Identifiers
DOI: 10.3791/62588
PMID: 33999017
Source
Medline
Language
English
License
Unknown

Abstract

Control of mosquito-borne pathogens using genetically-modified vectors has been proposed as a promising tool to complement conventional control strategies. CRISPR-based homing gene drive systems have made transgenic technologies more accessible within the scientific community. Evaluation of transgenic mosquito performance and comparisons with wild-type counterparts in small laboratory cage trials provide valuable data for the design of subsequent field cage experiments and experimental assessments to refine the strategies for disease prevention. Here, we present three different protocols used in laboratory settings to evaluate transgene spread in anopheline mosquito vectors of malaria. These include inundative releases (no gene-drive system), and gene-drive overlapping and non-overlapping generation trials. The three trials vary in a number of parameters and can be adapted to desired experimental settings. Moreover, insectary studies in small cages are part of the progressive transition of engineered insects from the laboratory to open field releases. Therefore, the protocols described here represent invaluable tools to provide empirical values that will ultimately aid field implementation of new technologies for malaria elimination.

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