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Small RNA sequencing reveals miRNAs important for hypoxic adaptation in the Tibetan chicken.

Authors
  • Zhang, Z1, 2
  • Qiu, M1
  • Du, H1
  • Li, Q1
  • Yu, C1
  • Gan, W3
  • Peng, H1
  • Xia, B1
  • Xiong, X1
  • Song, X1
  • Yang, L1
  • Hu, C1
  • Chen, J1
  • Yang, C1
  • Jiang, X1, 2
  • 1 Poultry Research Institute, Sichuan Animal Science Academy , Chengdu, Sichuan, China. , (China)
  • 2 Poultry Research Institute, Animal Breeding and Genetics Key Laboratory of Sichuan Province , Chengdu, Sichuan, China. , (China)
  • 3 Poultry Research Institute, Shanghai Ying Biotechnology Company , Shanghai, China. , (China)
Type
Published Article
Journal
British Poultry Science
Publisher
Informa UK (Taylor & Francis)
Publication Date
Dec 01, 2020
Volume
61
Issue
6
Pages
632–639
Identifiers
DOI: 10.1080/00071668.2020.1792835
PMID: 32631087
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

1. The Tibetan chicken, which is an indigenous breed living on the Tibetan Plateau, exhibits hypoxic adaptations to its high-altitude environment. However, the molecular mechanism behind this hypoxic adaptation is still unclear. This study aimed to investigate differentially expressed miRNAs involved in hypoxic adaptation through high-throughput RNA sequencing. 2. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the differentially expressed miRNAs and their target genes in chicken embryonic heart tissues and fibroblasts. Luciferase reporter assays were performed to confirm the relationship between miRNAs and target genes. 3. The study identified 37 differentially expressed miRNAs in Tibetan chicken embryonic heart tissues, including 20 up- and 17 down-regulated miRNAs, compared to those found in lowland chickens. Differentially expressed miRNAs were mainly involved in biological processes, such as cell cycle arrest, toll-like receptor signalling pathways, and I-kappa B kinase/NF-kappa B signalling. The data showed that gga-miR-34 c-5p was significantly upregulated in Tibetan chicken tissues and hypoxic fibroblasts, while EHHADH, a target gene of gga-miR-34 c-5p, was downregulated. Moreover, gga-miR-34 c-5p dramatically decreased the luciferase activity of the wild EHHADH, whereas no effect on the mutational EHHADH was found. 4. This study identified miRNA expression profiles in the Tibetan chicken and suggested that miR-34 c-5p acts as a novel miRNA associated with hypoxic adaptation. This facilitates the understanding of molecular mechanisms that underlie long-term exposure to hypoxia.

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