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Small Intestinal Pro-Inflammatory Immune Responses Following Campylobacter Jejuni Infection of Secondary Abiotic IL-10-/- Mice Lacking Nucleotide-Oligomerization-Domain-2.

Authors
  • Heimesaat, Markus M1
  • Grundmann, Ursula1
  • Alutis, Marie E1
  • Fischer, André1
  • Bereswill, Stefan1
  • 1 Department of Microbiology and Hygiene, Charité - University Medicine Berlin, Berlin, Germany. , (Germany)
Type
Published Article
Journal
European journal of microbiology & immunology
Publication Date
Jun 01, 2017
Volume
7
Issue
2
Pages
138–145
Identifiers
DOI: 10.1556/1886.2017.00005
PMID: 28690881
Source
Medline
Keywords
License
Unknown

Abstract

Host immune responses are crucial for combating enteropathogenic infections including Campylobacter jejuni. Within 1 week following peroral C. jejuni infection, secondary abiotic IL-10-/- mice develop severe immunopathological sequelae affecting the colon (ulcerative enterocolitis). In the present study, we addressed whether pathogen-induced pro-inflammatory immune responses could also be observed in the small intestines dependent on the innate receptor nucleotide-oligomerization-domain-protein 2 (Nod2). Within 7 days following peroral infection, C. jejuni stably colonized the gastrointestinal tract of both IL-10-/- mice lacking Nod2 (Nod2-/- IL-10-/-) and IL-10-/- controls displaying bloody diarrhea with similar frequencies. Numbers of apoptotic and regenerating epithelial cells increased in the small intestines of C. jejuni-infected mice of either genotype that were accompanied by elevated ileal T and B lymphocyte counts. Notably, ileal T cell numbers were higher in C. jejuni-infected Nod2-/- IL-10-/- as compared to IL-10-/- counterparts. Furthermore, multifold increased concentrations of pro-inflammatory cytokines including IFN-γ, TNF, and MCP-1 could be measured in small intestinal ex vivo biopsies derived from C. jejuni-infected mice of either genotype. In conclusion, C. jejuni-induced pro-inflammatory immune responses affected the small intestines of both Nod2-/- IL-10-/- and IL-10-/- mice, whereas ileal T lymphocyte numbers were even higher in the former.

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