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Skin mast cells develop non-synchronized changes in typical lineage characteristics upon culture.

Authors
  • Guhl, Sven1
  • Neou, Angelos
  • Artuc, Metin
  • Zuberbier, Torsten
  • Babina, Magda
  • 1 Department of Dermatology and Allergy, Charité Universitätsmedizin Berlin, Berlin, Germany. , (Germany)
Type
Published Article
Journal
Experimental Dermatology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Dec 01, 2014
Volume
23
Issue
12
Pages
933–935
Identifiers
DOI: 10.1111/exd.12558
PMID: 25271543
Source
Medline
Keywords
License
Unknown

Abstract

Despite their hematopoietic origin, mast cells (MCs) develop exclusively in tissues, hampering their ample use in research. To circumvent this problem, tissue-derived MCs are typically first expanded in culture, but the changes MCs may undergo in the novel micromilieu are poorly defined. Here, we monitor skin MCs from a number of donors over time, revealing profound yet non-synchronized modulations in culture. While tryptase and chymase, the most specific markers, strongly decline, FcεRI surface expression, and FcεRI-mediated histamine release steeply increase (from ≈15.5% to ≈60%), replicated by similar increments in TNF-α secretion. Interestingly, the modulations are independent of cell cycle progression, as they are comparable in the growth and postgrowth phase, implying they primarily result from microenvironmental conditioning. The data highlight a high degree of MC versatility, but also advise that results based on cultured MCs should be viewed with some caution, as they may not accurately reflect their counterparts in situ.

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