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Size and shape of poly(ADP-ribose): examination by gel filtration, gel electrophoresis and electron microscopy.

Authors
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
Volume
112
Issue
1
Pages
102–107
Identifiers
PMID: 6838598
Source
Medline
License
Unknown

Abstract

Digestion of the poly(ADP-ribose) with snake venom phosphodiesterase yielded phosphoribosyl-AMP and AMP in a ratio of 30 to 1, indicating the "chain length" of 30 if the poly(ADP-ribose) is a linear molecule as previously supposed. Gel filtration and gel electrophoresis, however, revealed a very heterogeneous size distribution, the average size being, much longer than (ADP-ribose)30. Electron microscopy of poly(ADP-ribose) showed an irregularly branched figure. Molecules with more than ten branched portions were observed. These results are in agreement with the recent identification of the chemical structure of the branched portion in poly(ADP-ribose).

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