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In Situ SDS-PAGE Peptide-Mapping Procedure (Cleveland Method).

Authors
Type
Published Article
Journal
Cold Spring Harbor Protocols
1559-6095
Publisher
Cold Spring Harbor Laboratory
Publication Date
Volume
2007
Identifiers
DOI: 10.1101/pdb.prot4570
PMID: 21357012
Source
Medline
License
Unknown

Abstract

INTRODUCTIONIn this protocol, sample proteins are first separated by either one- or two-dimensional gel electrophoresis. The gel is briefly stained and destained, and the proteins are excised for peptide mapping. Peptide fragmentation (either enzymatically or chemically) is performed in situ in the gel pieces, and the partial digest is separated in a second gel.

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