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In situ microscopy for in-line monitoring of the enzymatic hydrolysis of cellulose.

Authors
  • Opitz, Britta
  • Prediger, Andreas
  • Lüder, Christian
  • Eckstein, Marrit
  • Hilterhaus, Lutz
  • Lindner, Patrick
  • Beutel, Sascha
  • Scheper, Thomas
  • Liese, Andreas
Type
Published Article
Journal
Analytical Chemistry
Publisher
American Chemical Society
Publication Date
Sep 03, 2013
Volume
85
Issue
17
Pages
8121–8126
Identifiers
DOI: 10.1021/ac4008495
PMID: 23889679
Source
Medline
License
Unknown

Abstract

A new in-line method for the monitoring of enzymatic hydrolysis of cellulose is described. Using a new in situ microscope prototype, the noninvasive determination of particle size distributions was possible. For the automated analysis of the acquired images, a new processing algorithm called CelluloseAnalyzer was developed. It enabled tracking of the number of particles and moreover allowed monitoring of the proportions of particle size fractions during the course of enzymatic hydrolysis reactions. Using this technique, significant differences between hydrolysis with endoglucanases and cellulase mixtures were observed. Furthermore, the in situ microscopy results were compared with results from off-line measurements with laser diffraction spectroscopy and gel permeation chromatography.

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