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SitA contributes to the virulence of Klebsiella pneumoniae in a mouse infection model

Authors
  • Sun, Wei-Sheng W.
  • Syu, Wan-Jr
  • Ho, Wen-Li
  • Lin, Ching-Nan
  • Tsai, Shih-Feng
  • Wang, Shao-Hung1, 2, 3, 2, 4, 5, 6, 7, 8, 9
  • 1 Institute of Biochemistry and Molecular Biology
  • 2 National Yang-Ming University
  • 3 Institute of Microbiology and Immunology
  • 4 Taiwan International Graduate Program in Molecular Medicine
  • 5 Academia Sinica
  • 6 Institute of Molecular and Genomic Medicine
  • 7 National Health Research Institutes
  • 8 Department of Microbiology, Immunology and Biopharmaceuticals
  • 9 National Chiayi University
Type
Published Article
Journal
Microbes and Infection
Publisher
Elsevier
Publication Date
Jan 01, 2013
Accepted Date
Oct 30, 2013
Volume
16
Issue
2
Pages
161–170
Identifiers
DOI: 10.1016/j.micinf.2013.10.019
Source
Elsevier
Keywords
License
Unknown

Abstract

Klebsiella pneumoniae is an opportunistic pathogen, which causes a wide range of nosocomial infections. Recently, antibiotic resistance makes K. pneumoniae infection difficult to deal with. Investigation on virulence determinants of K. pneumoniae can provide more information about pathogenesis and unveil new targets for treatment or vaccine development. In this study, SitA, a Fur-regulated divalent cation transporter, was found significantly increased when K. pneumoniae was cultured in a nutrient-limited condition. A sitA-deletion strain (ΔsitA) was created to characterize the importance of SitA in virulence. ΔsitA showed higher sensitivity toward hydroperoxide than its parental strain. In a mouse intraperitoneal infection model, the survival rate of mice infected with ΔsitA strain increased greatly when compared with that of mice infected with the parental strain, suggesting that sitA deletion attenuates the bacterial virulence in vivo. To test whether ΔsitA strain is a potential vaccine candidate, mice were immunized with inactivated bacteria and then challenged with the wild-type strain. The results showed that using ΔsitA mutant protected mice better than using the wild-type strain or the capsule-negative congenic bacteria. In summary, SitA was found being important for the growth of K. pneumoniae in vivo and deleting sitA might be a potential approach to generate vaccines against K. pneumoniae.

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