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Single-stage autotrophic nitrogen-removal process using a composite matrix immobilizing nitrifying and sulfur-denitrifying bacteria

Authors
  • Aoi, Y.1
  • Shiramasa, Y.1
  • Kakimoto, E.1
  • Tsuneda, S.1
  • Hirata, A.1
  • Nagamune, T.2
  • 1 Waseda University, Department of Chemical Engineering, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo, 169-8555, Japan , Tokyo
  • 2 The University of Tokyo, Department of Chemistry and Biotechnology, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan , Tokyo
Type
Published Article
Journal
Applied Microbiology and Biotechnology
Publisher
Springer-Verlag
Publication Date
Feb 04, 2005
Volume
68
Issue
1
Pages
124–130
Identifiers
DOI: 10.1007/s00253-005-1910-9
Source
Springer Nature
Keywords
License
Yellow

Abstract

We developed a novel single-stage autotrophic nitrogen-removal process comprised of two composite immobilized biomass layers—one of nitrifying bacteria and one of sulfur-denitrifying bacteria and elemental sulfur—in a Fe-Ni fibrous slag matrix. Nitrification and consumption of dissolved oxygen occurred in the outer part and sulfur denitrification in the anoxic inner part of the composite matrix, thus realizing autotrophic nitrogen removal in a single reactor. The complete conversion of ammonia into N2 in a single reactor was demonstrated in both batch-mode incubation and continuous-feed operation. The spatial profiles of the ammonia-oxidizing bacteria and denitrifying bacteria were evaluated by real-time PCR, targeting their functional genes, and stratification of these two types was observed in the matrix after several months of incubation. This process does not require any specific reactor type or conditions and thus has the potential to be applied to many different wastewater treatment processes due to its simplicity in both operation and construction.

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