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Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures

Authors
  • Ramírez, Nadia1
  • Lorenzo, Damaris1
  • Palenzuela, Daniel2
  • Herrera, Lissett3
  • Ayala, Marta4
  • Fuentes, Alejandro1
  • Pérez, Marlene1
  • Gavilondo, Jorge4
  • Oramas, Pedro1
  • 1 Plant Division, Cuba
  • 2 Diagnostics Division, Cuba
  • 3 Bioplants Center, Ciego de Avila, Cuba , Ciego de Avila
  • 4 Center for Genetic Engineering and Biotechnology, Pharmaceuticals Division, Havana, Cuba , Havana
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Aug 01, 2000
Volume
22
Issue
15
Pages
1233–1236
Identifiers
DOI: 10.1023/A:1005605214703
Source
Springer Nature
Keywords
License
Yellow

Abstract

An anti-hepatitis B surface antigen (HBsAg), single-chain Fv antibody fragment (scFv) with a 6-histidine N-terminal tag was produced in cultured transgenic tobacco cells. Western blot and antigen-specific chromatography showed high levels of biologically active scFv in the culture supernatant (1 mg l−1) and in cells (5 mg kg−1). A simple one-step scFv purification was developed using immobilized metal ion affinity chromatography.

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