Affordable Access

Single and multiple ion recording techniques for the analysis of diphenylhydantoin and its major metabolite in plasma.

Authors
Type
Published Article
Journal
Biomedical mass spectrometry
Publication Date
Volume
4
Issue
1
Pages
36–41
Identifiers
PMID: 836941
Source
Medline

Abstract

A method has been developed for single ion monitoring of diphenylhydantoin and its major metabolite 5-(p-hydroxyphenyl)-5-phenylhydantoin in plasma. A plasma extract is reacted with N,O-bis(trimethylsilyl) acetamide and single ion recording is carried out using a gas chromatograph mass spectrometer system. The mass value selected, m/e 254, is common to the TMS ethers of diphenylhydantoin and its principal metabolite 5-(p-hydroxyphenyl)-5-phenylhydantoin. The result indicate that one cause of an adverse reaction to diphenylhydantoin could be a reduced ability to hydroxylate the drugmquantitative methods for the analysis of the drug and its major metabolite have also been developed. Diphenylhydantoin and 5-(p-hydroxyphenyl)-5-phenylhydantoin can be analysed in plasma after addition of deuterium labelled internal standards and conversion to volatile derivatives for mass fragmentographic analysis. Diphenylhydantoin and its internal standard are analysed as the N,N-dimethyl derivative, and the hydroxylated metabolite and its internal standard are converted to a petrimethylsilyl compound by reaction with N,O-bis-(trimethylsilyl)acetamide.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments
F