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Single-molecule emulsion PCR in microfluidic droplets.

Authors
  • Zhu, Zhi
  • Jenkins, Gareth
  • Zhang, Wenhua
  • Zhang, Mingxia
  • Guan, Zhichao
  • Yang, Chaoyong James
Type
Published Article
Journal
Analytical and Bioanalytical Chemistry
Publisher
Springer-Verlag
Publication Date
Jun 01, 2012
Volume
403
Issue
8
Pages
2127–2143
Identifiers
DOI: 10.1007/s00216-012-5914-x
PMID: 22451171
Source
Medline
License
Unknown

Abstract

The application of microfluidic droplet PCR for single-molecule amplification and analysis has recently been extensively studied. Microfluidic droplet technology has the advantages of compartmentalizing reactions into discrete volumes, performing highly parallel reactions in monodisperse droplets, reducing cross-contamination between droplets, eliminating PCR bias and nonspecific amplification, as well as enabling fast amplification with rapid thermocycling. Here, we have reviewed the important technical breakthroughs of microfluidic droplet PCR in the past five years and their applications to single-molecule amplification and analysis, such as high-throughput screening, next generation DNA sequencing, and quantitative detection of rare mutations. Although the utilization of microfluidic droplet single-molecule PCR is still in the early stages, its great potential has already been demonstrated and will provide novel solutions to today's biomedical engineering challenges in single-molecule amplification and analysis.

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