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Single-file electrophoretic transport and counting of individual DNA molecules in surfactant nanotubes.

Authors
  • Tokarz, Michal
  • Akerman, Björn
  • Olofsson, Jessica
  • Joanny, Jean-Francois
  • Dommersnes, Paul
  • Orwar, Owe
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Jun 28, 2005
Volume
102
Issue
26
Pages
9127–9132
Identifiers
PMID: 15961544
Source
Medline
License
Unknown

Abstract

We demonstrate a complete nanotube electrophoresis system (nanotube radii in the range of 50 to 150 nm) based on lipid membranes, comprising DNA injection, single-molecule transport, and single-molecule detection. Using gel-capped electrodes, electrophoretic single-file transport of fluorescently labeled dsDNA molecules is observed inside nanotubes. The strong confinement to a channel of molecular dimensions ensures a detection efficiency close to unity and identification of DNA size from its linear relation to the integrated peak intensity. In addition to constituting a nanotechnological device for identification and quantification of single macromolecules or biopolymers, this system provides a method to study their conformational dynamics, reaction kinetics, and transport in cell-like environments.

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