Affordable Access

deepdyve-link
Publisher Website

Single-cell isolation using a DVD optical pickup.

Authors
  • Kasukurti, A
  • Potcoava, M
  • Desai, S A
  • Eggleton, C
  • Marr, D W M
Type
Published Article
Journal
Optics Express
Publisher
The Optical Society
Publication Date
May 23, 2011
Volume
19
Issue
11
Pages
10377–10386
Identifiers
DOI: 10.1364/OE.19.010377
PMID: 21643294
Source
Medline
License
Unknown

Abstract

A low-cost single-cell isolation system incorporating a digital versatile disc burner (DVD RW) optical pickup has been developed. We show that these readily available modules have the required laser power and focusing optics to provide a steady Gaussian beam capable of optically trapping micron-sized colloids and red blood cells. Utility of the pickup is demonstrated through the non-destructive isolation of such particles in a laminar-flow based microfluidic device that captures and translates single microscale objects across streamlines into designated channel exits. In this, the integrated objective lens focusing coils are used to steer the optical trap across the channel, resulting in the isolation of colloids and red blood cells using a very inexpensive off-the-shelf optical component.

Report this publication

Statistics

Seen <100 times