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Simultaneous measurement of pressure in the interstitium and the terminal lymphatics of the cat mesentery.

Authors
Type
Published Article
Journal
The Journal of Physiology
0022-3751
Publisher
Wiley Blackwell (Blackwell Publishing)
Publication Date
Volume
283
Pages
457–468
Identifiers
PMID: 722586
Source
Medline

Abstract

1. Simultaneous measurements of the pressure in terminal lymphatics and interstitial tissue have been made in the exteriorized cat mesentery superfused with either physiological salt solution (Krebs solution) or a water-immiscible fluorocarbon, FC-80. 2. The pressures within individual terminal lymphatics were measured using glass micropipettes attached to a servo pressure-measuring system. Tissue pressures were recorded using saline-filled cotton-wool wicks. 3. Mean pressure recorded in the terminal lymphatics of the Krebs-superfused mesentery were slightly above atmospheric (+0.2 mmHg, n = 45), while those recorded in the FC-80-superfused mesentery were slightly below atmospheric (-0.2 mmHg, n = 46). 4. Tissue pressures were also slightly subatmospheric in the in situ mesentery, and the recently exposed tissue. Continuous superfusion with Krebs solution caused the tissue pressure to rise to atmospheric pressure or above; with FC-80-superfusion the tissue pressure also rose, but never to above atmospheric pressure. 5. Isolated strips of mesentery immersed in Krebs solutions of different concentrations gained weight, but when immersed in FC-80 no change in weight was detected. 6. It was concluded that the interstitial gel of the mesentery is normally unsaturated and that superfusion with Krebs solution leads to tissue oedema. This tendency is less marked in FC-80-superfused preparations. Possible mechanisms for lymph formation and propulsion are discussed.

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