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Simultaneous measurement of bone resorption and collagen synthesis in neonatal mouse calvaria

Authors
  • Hefley, Thomas J.
  • Krieger, Nancy S.
  • Stern, Paula H.
Type
Published Article
Journal
Analytical Biochemistry
Publication Date
Jan 01, 1986
Volume
153
Issue
1
Pages
166–171
Identifiers
DOI: 10.1016/0003-2697(86)90076-X
Source
Elsevier
Keywords
License
Unknown

Abstract

A tissue culture technique which permits the simultaneous measurement of collagen synthesis and bone resportion has been developed. Cultured neonatal mouse calvaria undergo resorption when stimulated by a number of agents including parathyroid hormone, vitamin D, and prostaglandin E 2. Mouse calvaria are of sufficient size to measure the extent of proline incorporation into collagenase-digestible protein. Four chemically diverse stimulators of bone resorption were tested for their effect on collagen synthesis. For each stimulator tested, the dose-response relationships for the stimulation of resorption and the inhibition of collagen synthesis were found to coincide.

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