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Simultaneous estimation of etoricoxib and thiocolchicoside by RP-HPLC method in combined dosage forms.

Authors
  • Kumar, Sanjiv
  • Joshi, Amit
  • Thakur, Rahul Singh
  • Pathak, Anupam K
  • Shah, Kamal
Type
Published Article
Journal
Acta poloniae pharmaceutica
Publication Date
Jan 01, 2011
Volume
68
Issue
6
Pages
839–843
Identifiers
PMID: 22125947
Source
Medline
License
Unknown

Abstract

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of etoricoxib and thiocolchicoside in combined tablet dosage form. Formulation containing etoricoxib and thiocolchicoside is used as analgesic. Chromatography was performed on a 250 mm x 4.6 mm, 5-microm particle size, BDS Hypersil C-18 column with trifluoroacetic acid buffer (pH 2.6) and acetonitrile (75:25, v/v) as a mobile phase. The detection of the combined dosage form was carried out at 220 nm and a flow rate employed was 1.5 mL/min. The retention times were 6.6 and 3.1 min for etoricoxib and thiocolchicoside, respectively. Linearity was obtained in the concentration range 20 to 160 ppm for etoricoxib and in the range 2 to 16 ppm for thiocolchicoside with a correlation coefficient of 0.9918 and 0.9994, respectively. The results of the analysis were validated statistically and recovery studies confirmed the accuracy and precision of the proposed method.

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