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Simultaneous detection of Plasmodium vivax dhfr, dhps, mdr1 and crt-o resistance-associated mutations in the Colombian Amazonian region

Authors
  • Cubides, Juan Ricardo1
  • Camargo-Ayala, Paola Andrea1
  • Niño, Carlos Hernando1
  • Garzón-Ospina, Diego1, 2
  • Ortega-Ortegón, Anggie1
  • Ospina-Cantillo, Estefany1
  • Orduz-Durán, María Fernanda1
  • Patarroyo, Manuel Elkin1, 3
  • Patarroyo, Manuel Alfonso1, 2
Type
Published Article
Journal
Malaria Journal
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Mar 26, 2018
Volume
17
Issue
1
Identifiers
DOI: 10.1186/s12936-018-2286-5
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundMalaria continues being a public health problem worldwide. Plasmodium vivax is the species causing the largest number of cases of malaria in Asia and South America. Due to the lack of a completely effective anti-malarial vaccine, controlling this disease has been based on transmission vector management, rapid diagnosis and suitable treatment. However, parasite resistance to anti-malarial drugs has become a major yet-to-be-overcome challenge. This study was thus aimed at determining pvmdr1, pvdhfr, pvdhps and pvcrt-o gene mutations and haplotypes from field samples obtained from an endemic area in the Colombian Amazonian region.MethodsFifty samples of parasite DNA infected by a single P. vivax strain from symptomatic patients from the Amazonas department in Colombia were analysed by PCR and the pvdhfr, pvdhps, pvmdr1 and pvcrt-o genes were sequenced. Diversity estimators were calculated from the sequences and the haplotypes circulating in the Colombian Amazonian region were obtained.Conclusionpvdhfr, pvdhps, pvmdr1 and pvcrt-o genes in the Colombian Amazonian region are characterized by low genetic diversity. Some resistance-associated mutations were found circulating in this population. New variants are also being reported. A selective sweep signal was located in pvdhfr and pvmdr1 genes, suggesting that these mutations (or some of them) could be providing an adaptive advantage.

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