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Simultaneous analysis of homocysteine and methionine in plasma.

Authors
Type
Published Article
Journal
Journal of chromatography. B, Biomedical sciences and applications
Publication Date
Volume
759
Issue
1
Pages
145–151
Identifiers
PMID: 11499618
Source
Medline
License
Unknown

Abstract

The new isocratic cation exchange method separates up to eight different amino thiols. The separated sample components are detected electrochemically using a gold electrode and the integrated pulsed amperometry. The eluent composition is, for example, 0.15 M sodium perchlorate, 0.02 M perchloric acid and 5% acetonitrile. The report describes the optimization of chromatographic parameters such as column diameter and eluent composition. Quantitative performance is discussed for eight different amino thiols using standards. Also presented is a long term quantitative study for homocysteine and methionine in plasma samples. The preparation of plasma samples is simpler than with the previously reported version of the method. Only a reduction step is required, and neither column switching nor derivatization are necessary.

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