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Simple technique for the direct isolation of toxoplasma tissue cysts from fetal ovine brain.

Authors
Type
Published Article
Journal
The Veterinary record
Publication Date
Volume
112
Issue
5
Pages
98–100
Identifiers
PMID: 6829161
Source
Medline
License
Unknown

Abstract

A simple technique is described for the isolation of Toxoplasma gondii tissue cysts from fetal ovine brain by centrifugation on a discontinuous density gradient of 30 per cent and 90 per cent Percoll (colloidal silica solution). Brain samples from 51 aborted ovine fetuses were examined by both the Percoll and mouse inoculation techniques; eight infections were detected by the Percoll technique compared to 12 by mouse inoculation. Possible reasons for this discrepancy and the scope for improving the Percoll technique are discussed.

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